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phosphate buffer solution pbs  (Thermo Fisher)


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    Thermo Fisher phosphate buffer solution pbs
    Phosphate Buffer Solution Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphate+buffered+saline+solution/Phosphate-buffered+saline+(PBS%2C+10X)%2C+pH+7%2E4%2C+for+Western+blot/pm42308603-67-14-21
    Average 97 stars, based on 1 article reviews
    phosphate buffer solution pbs - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Saline:

    Article Title: Metasurface analogues of molecular diastereomers from hierarchical multiscale chiral interactions with biomolecules.
    Article Snippet: .. Phosphate buffered saline solution of pH 7.4 (10010031) was purchased from Gibco. ..

    Article Title: A "DUBTAC" targeting GLUL deubiquitination promotes BMSC osteogenic differentiation and implant osseointegration in type 2 diabetes.
    Article Snippet: .. After centrifugation at 1100 rpm in phosphate-buffered saline solution (Gibco, Grand Island, NY, USA), the bone fragments were resuspended in mesenchymal stem cell culture medium (MSCM; ScienCell, Carlsbad, CA, USA), inoculated into culture dishes, and cultured for 7 days in a humidified cell culture incuba- tor containing 5% CO2 at 37 °C without movement. .. Single-cell suspensions were obtained using a 70-lm pore size filter (Falcon, BD Labware, Wilmington, DE, USA) and then cultured in MSCM.

    Article Title: Fluvastatin and vascular endothelial growth factor Containing Resins Promote Angiogenesis and Arteriogenesis.
    Article Snippet: Introduction: Many patients with chronic limb threatening ischemia rely on neovascularization.. Statins and vascular endothelial growth factor (VEGF) stimulate neovascularization.. We hypothesized that locally delivered TentaGel (TG) resins containing VEGF-165A or fluvastatin would increase neovascularization in hindlimb ischemia and their combination would be most effective.

    Article Title: Naproxen and Ibuprofen Exposure Alter the Blood-Testis Barrier in a Novel In Vitro Model.
    Article Snippet: .. NHP primary Sertoli cells cultured in the conditions described above were fixed in 4% paraformaldehyde (Cat. No. 15710, Electron Microscopy Solutions, Hatfield, PA, USA) for 15 min, then blocked with buffer containing 1× Phosphate-buffered Saline solution (PBS, Cat. No. 10010023, Gibco, USA), 0.1% Triton X (Cat. No. X100, Sigma, USA), and 5% normal goat serum (Cat. No. 092939149, MP Biomedicals, Santa Ana, CA, USA) or normal donkey serum (Cat. No. 017-000-121, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) overnight at 4 ◦C as described [83]. .. For primary antibodies, SOX9 and WT1 (Table S2 and Figure S5B), incubation was performed overnight at 4 ◦C in blocking buffer, followed by three washes in 1X PBS with 0.1% Triton X for 10 min each at room temperature (RT) on an LSE Orbital Shaker (Corning®).

    Article Title: Carbon Nanotube Hydrogel Electrodes for High-Fidelity Intra-Aural EEG in Wearable Neurotechnology
    Article Snippet: .. Two types of EIS measurements were performed: (i) In liquid, using a three-electrode assembly consisting of a reference electrode (Ag/AgCl), a counter electrode (Pt wire), and the working electrode (hydrogel connected via a cable with alligator clips); the supporting electrolyte is a phosphate-buffered saline solution, pH = 7.4, from Alfa Aesar (Thermo Scientific Chemicals, Waltham, MA, USA). (ii) Under dry conditions, using an adjustable 2-electrode platform designed for EIS measurements (a hollow round perfluoroalkoxy chamber equipped with 2 round steel electrodes connected to the Origalys potentiostat via simple connecting cables; the counter electrode and the reference electrode are electrically coupled; the hydrogel sample is placed between the two electrodes). ..

    Article Title: A multi-modal strategy for enhancing titanium–bone interface strength and antimicrobial properties of PMMA bone cements
    Article Snippet: .. On the day of experiment, the cells were washed with 4 mL of Dulbecco’s phosphate buffered saline solution twice (Thermo fisher scientific, Waltham, MA, USA), followed by detaching the cells from the culture flask using 5 mL of TrypLE TM Express Enzyme (Gibco, Thermo fisher scientific). .. Cells were counted using a Countess II Automated Cell Counter (Thermo fisher scientific) then 10,000 cells per well were seeded in a 96-well plate and incubated for 24 h to reach 90% confluency.

    Article Title: Prediction of personalized therapeutic response in pediatric clivus chordoma: chemoresistance test.
    Article Snippet: .. To confirm the cytotoxic effect, clival chordoma cells were incubated with DMEM-LG (control) and irinotecan at concentrations CP-11 D1 [50 μM] and CP-11 D2 [110 μM] for 72 h. After washing with cold phosphate-buffered saline solution, 105 cells were resuspended in 100 μL of 1X binding buffer (Molecular Probes, Invitrogen) and labeled with 5 μL FITC-Annexin V and 5 μL propidium iodide (PI) (BD Biosciences). .. The samples were incubated for 15 min in the dark at room temperature, brought to 500 μL with 1X binding buffer, and analyzed on an LSR Fortessa flow cytometer (BD Biosciences) within 1 h. Data analysis was performed using the FlowJo software.

    Article Title: In vitro investigation of electrospun PVDF-TrFE fiber mats to reduce connective tissue growth after cochlear implantation
    Article Snippet: To examine the cell response to the polymeric structure, an extract was prepared by incubating the fiber mat in cell specific culture medium (Dulbecco’s Modified Eagle’s Medium (DMEM) (Bio&Sell, Berlin, Germany) for fibroblasts and supplemented Panserin 401 (Pan Biotech, Passau, Germany) for SGNs. .. Therefore, rectangular samples measuring 12 cm 2 (2 × 6 cm) were prepared from the fiber mat of 30 min spinning time and sterilized by washing them once in 70% ethanol and twice in phosphate buffered saline solution (PBS; Invitrogen, Karlsruhe, Germany). ..

    Centrifugation:

    Article Title: A "DUBTAC" targeting GLUL deubiquitination promotes BMSC osteogenic differentiation and implant osseointegration in type 2 diabetes.
    Article Snippet: .. After centrifugation at 1100 rpm in phosphate-buffered saline solution (Gibco, Grand Island, NY, USA), the bone fragments were resuspended in mesenchymal stem cell culture medium (MSCM; ScienCell, Carlsbad, CA, USA), inoculated into culture dishes, and cultured for 7 days in a humidified cell culture incuba- tor containing 5% CO2 at 37 °C without movement. .. Single-cell suspensions were obtained using a 70-lm pore size filter (Falcon, BD Labware, Wilmington, DE, USA) and then cultured in MSCM.

    Stem Cell Culture:

    Article Title: A "DUBTAC" targeting GLUL deubiquitination promotes BMSC osteogenic differentiation and implant osseointegration in type 2 diabetes.
    Article Snippet: .. After centrifugation at 1100 rpm in phosphate-buffered saline solution (Gibco, Grand Island, NY, USA), the bone fragments were resuspended in mesenchymal stem cell culture medium (MSCM; ScienCell, Carlsbad, CA, USA), inoculated into culture dishes, and cultured for 7 days in a humidified cell culture incuba- tor containing 5% CO2 at 37 °C without movement. .. Single-cell suspensions were obtained using a 70-lm pore size filter (Falcon, BD Labware, Wilmington, DE, USA) and then cultured in MSCM.

    Cell Culture:

    Article Title: A "DUBTAC" targeting GLUL deubiquitination promotes BMSC osteogenic differentiation and implant osseointegration in type 2 diabetes.
    Article Snippet: .. After centrifugation at 1100 rpm in phosphate-buffered saline solution (Gibco, Grand Island, NY, USA), the bone fragments were resuspended in mesenchymal stem cell culture medium (MSCM; ScienCell, Carlsbad, CA, USA), inoculated into culture dishes, and cultured for 7 days in a humidified cell culture incuba- tor containing 5% CO2 at 37 °C without movement. .. Single-cell suspensions were obtained using a 70-lm pore size filter (Falcon, BD Labware, Wilmington, DE, USA) and then cultured in MSCM.

    Article Title: Naproxen and Ibuprofen Exposure Alter the Blood-Testis Barrier in a Novel In Vitro Model.
    Article Snippet: .. NHP primary Sertoli cells cultured in the conditions described above were fixed in 4% paraformaldehyde (Cat. No. 15710, Electron Microscopy Solutions, Hatfield, PA, USA) for 15 min, then blocked with buffer containing 1× Phosphate-buffered Saline solution (PBS, Cat. No. 10010023, Gibco, USA), 0.1% Triton X (Cat. No. X100, Sigma, USA), and 5% normal goat serum (Cat. No. 092939149, MP Biomedicals, Santa Ana, CA, USA) or normal donkey serum (Cat. No. 017-000-121, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) overnight at 4 ◦C as described [83]. .. For primary antibodies, SOX9 and WT1 (Table S2 and Figure S5B), incubation was performed overnight at 4 ◦C in blocking buffer, followed by three washes in 1X PBS with 0.1% Triton X for 10 min each at room temperature (RT) on an LSE Orbital Shaker (Corning®).

    Incubation:

    Article Title: Fluvastatin and vascular endothelial growth factor Containing Resins Promote Angiogenesis and Arteriogenesis.
    Article Snippet: Introduction: Many patients with chronic limb threatening ischemia rely on neovascularization.. Statins and vascular endothelial growth factor (VEGF) stimulate neovascularization.. We hypothesized that locally delivered TentaGel (TG) resins containing VEGF-165A or fluvastatin would increase neovascularization in hindlimb ischemia and their combination would be most effective.

    Article Title: Prediction of personalized therapeutic response in pediatric clivus chordoma: chemoresistance test.
    Article Snippet: .. To confirm the cytotoxic effect, clival chordoma cells were incubated with DMEM-LG (control) and irinotecan at concentrations CP-11 D1 [50 μM] and CP-11 D2 [110 μM] for 72 h. After washing with cold phosphate-buffered saline solution, 105 cells were resuspended in 100 μL of 1X binding buffer (Molecular Probes, Invitrogen) and labeled with 5 μL FITC-Annexin V and 5 μL propidium iodide (PI) (BD Biosciences). .. The samples were incubated for 15 min in the dark at room temperature, brought to 500 μL with 1X binding buffer, and analyzed on an LSR Fortessa flow cytometer (BD Biosciences) within 1 h. Data analysis was performed using the FlowJo software.

    Electron Microscopy:

    Article Title: Naproxen and Ibuprofen Exposure Alter the Blood-Testis Barrier in a Novel In Vitro Model.
    Article Snippet: .. NHP primary Sertoli cells cultured in the conditions described above were fixed in 4% paraformaldehyde (Cat. No. 15710, Electron Microscopy Solutions, Hatfield, PA, USA) for 15 min, then blocked with buffer containing 1× Phosphate-buffered Saline solution (PBS, Cat. No. 10010023, Gibco, USA), 0.1% Triton X (Cat. No. X100, Sigma, USA), and 5% normal goat serum (Cat. No. 092939149, MP Biomedicals, Santa Ana, CA, USA) or normal donkey serum (Cat. No. 017-000-121, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) overnight at 4 ◦C as described [83]. .. For primary antibodies, SOX9 and WT1 (Table S2 and Figure S5B), incubation was performed overnight at 4 ◦C in blocking buffer, followed by three washes in 1X PBS with 0.1% Triton X for 10 min each at room temperature (RT) on an LSE Orbital Shaker (Corning®).

    Impedance Spectroscopy:

    Article Title: Carbon Nanotube Hydrogel Electrodes for High-Fidelity Intra-Aural EEG in Wearable Neurotechnology
    Article Snippet: .. Two types of EIS measurements were performed: (i) In liquid, using a three-electrode assembly consisting of a reference electrode (Ag/AgCl), a counter electrode (Pt wire), and the working electrode (hydrogel connected via a cable with alligator clips); the supporting electrolyte is a phosphate-buffered saline solution, pH = 7.4, from Alfa Aesar (Thermo Scientific Chemicals, Waltham, MA, USA). (ii) Under dry conditions, using an adjustable 2-electrode platform designed for EIS measurements (a hollow round perfluoroalkoxy chamber equipped with 2 round steel electrodes connected to the Origalys potentiostat via simple connecting cables; the counter electrode and the reference electrode are electrically coupled; the hydrogel sample is placed between the two electrodes). ..

    Control:

    Article Title: Prediction of personalized therapeutic response in pediatric clivus chordoma: chemoresistance test.
    Article Snippet: .. To confirm the cytotoxic effect, clival chordoma cells were incubated with DMEM-LG (control) and irinotecan at concentrations CP-11 D1 [50 μM] and CP-11 D2 [110 μM] for 72 h. After washing with cold phosphate-buffered saline solution, 105 cells were resuspended in 100 μL of 1X binding buffer (Molecular Probes, Invitrogen) and labeled with 5 μL FITC-Annexin V and 5 μL propidium iodide (PI) (BD Biosciences). .. The samples were incubated for 15 min in the dark at room temperature, brought to 500 μL with 1X binding buffer, and analyzed on an LSR Fortessa flow cytometer (BD Biosciences) within 1 h. Data analysis was performed using the FlowJo software.

    Binding Assay:

    Article Title: Prediction of personalized therapeutic response in pediatric clivus chordoma: chemoresistance test.
    Article Snippet: .. To confirm the cytotoxic effect, clival chordoma cells were incubated with DMEM-LG (control) and irinotecan at concentrations CP-11 D1 [50 μM] and CP-11 D2 [110 μM] for 72 h. After washing with cold phosphate-buffered saline solution, 105 cells were resuspended in 100 μL of 1X binding buffer (Molecular Probes, Invitrogen) and labeled with 5 μL FITC-Annexin V and 5 μL propidium iodide (PI) (BD Biosciences). .. The samples were incubated for 15 min in the dark at room temperature, brought to 500 μL with 1X binding buffer, and analyzed on an LSR Fortessa flow cytometer (BD Biosciences) within 1 h. Data analysis was performed using the FlowJo software.

    Labeling:

    Article Title: Prediction of personalized therapeutic response in pediatric clivus chordoma: chemoresistance test.
    Article Snippet: .. To confirm the cytotoxic effect, clival chordoma cells were incubated with DMEM-LG (control) and irinotecan at concentrations CP-11 D1 [50 μM] and CP-11 D2 [110 μM] for 72 h. After washing with cold phosphate-buffered saline solution, 105 cells were resuspended in 100 μL of 1X binding buffer (Molecular Probes, Invitrogen) and labeled with 5 μL FITC-Annexin V and 5 μL propidium iodide (PI) (BD Biosciences). .. The samples were incubated for 15 min in the dark at room temperature, brought to 500 μL with 1X binding buffer, and analyzed on an LSR Fortessa flow cytometer (BD Biosciences) within 1 h. Data analysis was performed using the FlowJo software.



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